Fig. 6.

Expression of Rr-eng-1 in Rotylenchulus reniformis life-stages as determined by quantitative real-time PCR. Presented are the mean ± standard error fold expression values from three technical replicates of Rr-eng-1 relative to that in sedentary female nematodes (set = 1.0). cDNA was generated from R. reniformis eggs, J2, J3, J3+J4, adult vermiform (a mixture of males and pre-parasitic females), and sedentary females. Rr-eng-1 expression was normalized to the level of 18S rRNA in each sample. This experiment was repeated twice with similar results.