Figure 4. Carbamylation of HDL increases binding affinity to SR-BI.

THP1 macrophages infected with adenoviral vectors encoding SR-BI or LacZ (control) were incubated for 2 h at 4 °C in the presence of increasing concentrations of (A)¹²⁵I-labeled native HDL or ¹²⁵I-labeled carbamylated HDL (cHDL) or (B) ¹²⁵I-labeled reconstituted HDL (rHDL) or ¹²⁵I-labeled carbamylated rHDL. Values obtained with LacZ expressing cells were subtracted from SR-BI expressing cells to calculate SR-BI specific binding. Results represent the mean of triplicate determinations ± SD of a representative experiment performed at least two times.