Figure 6. Overexpression of Annexin A2–GFP rescues the endosomal localization of APPL2 in cells transfected with the dominant-negative Rab5-S34N mutant.

CCD-1070SK cells were transfected with plasmids encoding Annexin A2–GFP, myc-tagged Rab5-S34N or both, as indicated. Cells were fixed and immunostained with antibodies against APPL2 (green), GFP (red) and myc (blue). Single confocal sections are shown. Scale bar, 20 µm.