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. 2012 May 6;3(4):435–449. doi: 10.18632/oncotarget.483

Figure 6. AMPK activation by DIM is necessary for DIM induced autophagy.

Figure 6

A) Effect of various concentrations of DIM on p-AMPK (Ser 217) in i) SKOV-3, ii) OVCAR-3 or iii) TOV-21G cells. B) Representative blots of p-AMPK, Atg12, P62 or LC3B treated with 75μM DIM in presence or absence of 20μM Compound C in i) SKOV-3 or ii) OVCAR-3 cells. C) Silencing of AMPK using AMPK siRNA in SKOV-3 cells and treated with DIM for 24 hours. Whole cell lysates were analyzed by western blotting for the expression of p-AMPK, P62 and LC3B. Actin was used as a loading control.