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. 2012 May 6;3(4):435–449. doi: 10.18632/oncotarget.483

Figure 7. Increase in the cytosolic calcium plays a crucial role in DIM induced autophagy.

Figure 7

A) SKOV-3 (i), OVCAR-3 (ii) or TOV-21G (iii) cells were loaded with Fluo-3AM dye. Using a flowcytometer, base line reading was taken for a minute. Immediately, cells were exposed to 75 μM DIM for a minute followed by treatment with EGTA for another minute. Increase in the FL-1 intensity is related to increase in the cytosolic calcium levels. B) SKOV-3 (i) or OVCAR-3 (ii) cells were treated with BAPT-AM for one hour followed by treatment with 75μM DIM for 4 hours. Whole cell lysates were analyzed for western blotting for p-AMPK (Ser 217), Atg12 and LC3B. Actin was used as a loading control.