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. 2012 May 9;3(4):475–489. doi: 10.18632/oncotarget.479

Figure 4. Singular v dual knockdown of SNF2L and SNF2LT and DNA damage.

Figure 4

A, MDA-MB-468 cells were transfected with SNF2L siRNA, SNF2L siRNA or NCSI. 48 hours after transfection, DNA damage was analyzed by the Comet assay and the results showed damaged DNA (the comet tail) outside the nucleus after treatment of SNF2LT siRNA (lower panel), SNF2L siRNA (middle panel) compared to undamaged DNA in the cells treated with NCSI (upper panel). B, the surrogate DNA damage gene, p-H2AX showed increased expression following either SNF2L or SNF2LT knockdown (upper panel) and increased fold expression of p-H2AX (lower panel). Each experiment was performed in triplicate and repeated at least four times.