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. 2012 May 9;3(4):475–489. doi: 10.18632/oncotarget.479

Figure 6. Singular v dual knockdown of SNF2L and SNF2LT and apoptosis.

Figure 6

MDA-MB-468 cells were transfected with the different siRNAs. A, MDA-MB-468 cells were monitored over the next 48-72 hours with Annexin V-FITC and propidium iodide (PI) staining. The percentage of total apoptotic cells (early and late apoptosis) is depicted. Singular knockdowns of either SNF2L or SNF2LT both led to substantial increases in both early as well as late apoptosis. Dual knockdowns did not result in apoptosis over levels induced by the NCSI negative control (data not shown). During this time period the relative mRNA levels of potential genes involved in the apoptotic process was surveyed. B, singular knockdown of SNF2L led to a significant increase in Apaf-1 and caspase 9 whereas singular knockdown of SNF2LT led to a significant increase in caspase 8, BAD and BIK. C, dual knockdowns compared to NCSI knockdown did not result in a significant increase in any caspase. Each experiment was performed in triplicate and repeated at least four times.