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. 2012 May 14;110(1):47–56. doi: 10.1093/aob/mcs103

Fig. 5.

Fig. 5.

Western blot analysis of total proteins from apoplast of Brachypodium plants expressing microbial hydrolases. On the left are shown protein size markers (M). Corresponding microbial proteins fused with GFP were found in all Brachypodium plants selected by fluorescence: lane 1, a plant expressing an AnGAL–GFP fusion protein (62 kDa); lane 2, a plant expressing an AnRAE–GFP fusion protein (53 kDa); lane 3, a plant expressing an AnAF–GFP fusion protein (109 kDa). Proteins were detected using anti-GFP monoclonal antibodies (at a dilution of 1 : 5000).