Fig. 7.

(A) Acetyl content (μmol mg⁻¹ of dry cell wall material) in the cell wall of Brachypodium plants expressing AnRAE and of wild-type plants. Analyses were done using three independent leaves collected from two independent regenerated plants or wild-type plants grown simultaneously. Bars show the mean ± standard deviation. The difference between infiltrated and wild-type plants is not significant (t-test, n = 6, P > 0·05). (B) Amount of reducing sugars released after 8 h incubation of 10 mg of dry cell wall material with cellulase mixture (4 U of cellulase from T. reesei and 1 U of cellobiase from A. niger). *Difference is significant (t-test, P < 0·05; n = 6).