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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Oct 6;31(12):2889–2896. doi: 10.1161/ATVBAHA.111.236570

Figure 5.

Figure 5

Panel a shows Alexa 633 hydrazide staining of adventitial fibers in a cannulated cremaster 1A segment. Panel b shows the same vessel segment stained with a specific elastin antibody and a secondary antibody conjugated with Alexa fluor 488. Insert shows secondary antibody only control (additional details of controls are provided in the Supplementary Material). Panel c shows the overlay image for panels a and b. Results are typical of n = 4. Yellow color indicates apparent colocalization suggesting that the Alexa 633 hydrazide stained fibers contain elastin. Panel d elastase (0.05 U/ml, 5 min) cuts the longitudinally arranged, elastin-containing fibers (typical of n = 3).