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. Author manuscript; available in PMC: 2012 Jun 21.
Published in final edited form as: J Mol Biol. 2010 Dec 15;406(2):285–312. doi: 10.1016/j.jmb.2010.12.012

Figure 3. Thermodynamic pseudocycles.

Figure 3

(a) Equilibrium binding of unmodified DNA to wild type and mutant EcoRV; (b) Equilibrium binding of wild type EcoRV to unmodified and PMe modified DNA; (c) Equilibrium binding of mutant EcoRV to unmodified and PMe modified DNA. The free energy changes (ΔG°) for the reversible processes indicated by horizontal arrows can be determined by equilibrium binding experiments, but the “processes” indicated by vertical arrows represent chemical modifications or protein mutations, with free energy changes that are not experimentally accessible.