Fig. 1.
Effect of heat shock on expression of adenovirus-delivered EGFP and EGFP-mGluR1a. (A) CHO-K1 cells were infected one day before heating using different titers (MOI) of adenovirus and fluorescence of the expressed proteins was measured 24 hrs after HS treatment. (B) Time course of protein expression in CHO-K1 cells at different days after HS treatment, performed on day 0. (C) Effect of HS on adenovirus-mediated EGFP expression in different cell lines. CHO-K1 and PC-12 cells (infected at MOI 0.25), and HEK 293 cells (infected at MOI 0.015) were treated and monitored as in (B). Cells were heated for 2 hrs at 42.5°C.