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. Author manuscript; available in PMC: 2012 Jun 21.
Published in final edited form as: Neuropharmacology. 2011 Jan 15;60(7-8):1292–1300. doi: 10.1016/j.neuropharm.2011.01.010

Fig. 2.

Fig. 2

Effect of heat shock on the reporter gene expression driven by the CMV-IE promoter/enhancer in transfected CHO-K1 cells. CHO-K1 cells were permanently transfected with pEGFP-C2 vector expressing EGFP as a reporter gene under control of the CMV-IE promoter/enhancer. The cells were heated at 42.5°C for the indicated time. (A) Activity of the promoter was evaluated by measurement of the reporter gene expression (i.e. EGFP fluorescence) 24 hrs later. Cell viability was measured by MTT assay in the same samples. (B) Whole cell extracts were collected next day after heating. Samples (5 μg of total protein) were resolved on 12 % PAGE. EGFP expression was measured by Western blotting with specific anti-GFP antibodies. Each protein band corresponds to the time-point indicated in (A).