Figure 2.

TRPV4 and TRPP2 involvement in flow-induced [Ca²⁺]_i entry in M1-CCD cells. (A and C) Representative traces illustrating the time course of flow-induced [Ca²⁺]_i responses. (B and D) Summary showing the maximal change in flow-induced [Ca²⁺]_i responses (Δ[Ca²⁺]_i). In A and B, the cells were transfected with an empty plasmid vector (vector), TRPV4^M680D (V4^M680D), or TRPP2^D511V (P2^D511V). In C and D, the cells were pretreated with Preimmune IgG (Preimmu) or T1E3. Data are given as the mean ± SE (n=8–10 experiments and 10–20 cells per experiment). ^ΔP<0.01 versus vector.