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Apical mistargeting of mutant GFP-tagged kAE1-M909T in polarized MDCK cells. N-terminal GFP-tagged kAE1wt or kAE1-M909T (green) expressed in stably transfected MDCK-II cells grown to polarity on Transwells shows normal basolateral localization of kAE1wt and both apical and basolateral localization of kAE1-M909T confirmed by immunostaining in these nonpermeabalized cells with Bric-6 (antibody directed against an exofacial loop). Scale bars, 10 µm.
