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GFP-kAE1-M909A and -908stop mutants are intracellularly retarded. Both Bric-6 (columns 1–3, nonpermeabilized) and Bric-170 (columns 4–6, permeabilized) staining failed to show membrane localization of either mutant in mixed stable lines in contrast to normal localization of kAE1wt (top row). Scale bars, 20 µm.
