Fig. 2.

Expression of the myc17ER transgene in two medaka transgenic lines. (A) Real-time PCR analysis of the myc17ER transgene from liver, brain, eyes, gills and muscle from adult fish. Levels from the transgene were normalized against ef1a1 in each tissue. Histograms with T-bars indicate the mean standard deviation based on triplicate assays. (B) Western blot analysis of the Myc17ER fusion proteins. Protein samples extracted from pooled wild type (WT) and two transgenic fish lines were reacted with anti-ER antibody (30 hatched embryos per line). Protein extract containing human MycER (95 kDa), which has a higher molecular weight than medaka Myc17 (85 kDa), was used as control (crtl). Smaller and weaker bands in wild type control are due to unspecific antibody binding.