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Effects of myc17 activation on cell death in vitro. (A) Detection of apoptosis in primary cell culture (arrows indicate TUNEL-positive cells). Cells were treated for 5 hours with 4-OHT before fixation. Nuclei were counterstained with Hoechst. Scale bar: 100 μm. (B) Quantification of the percentage of apoptotic cells in primary cultures from both myc17ER lines and wild-type fish in the presence or absence of 4-OHT in the media. C N indicates total cell number for each assay.
