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Quantification of mRNA levels associated with the membrane protein genes in Salmonella. Bacteria were cultured in LB medium until mid-log phase (OD600=0.8). Fold change in the mRNA levels from the Δstn versus the parent strain was measured by quantitative real-time PCR. mRNA levels were normalized to 16S rRNA levels. Data are expressed as means ± s.d. values of five independent experiments.
