Fig. 2.

Side cross-sectional view of the microfluidic chamber (not drawn to scale). (a) Small quantities of oocytes are introduced into the inlet port and a syringe pump pulls the oocytes into the channels. The channel height is 86 µm, which allows for 60 µm diameter oocytes to flow through. Oocytes are stopped by a physical barrier that only allows filtered sea water to flow through a 26 µm high channel to the outlet port. (b) Oocytes are irradiated using a focused 337 nm UV beam. (c) 14% KCl/filtered sea water is perfused through the channel to activate the oocytes. (d) Fresh media is continuously changed using the syringe pump. Cells are checked for polar body ejections after 50 min.