Fig. 1.

Subcellular localization of Venus- and Cerulean-tagged versions of ${\mathrm{Atg4B}}^{\mathrm{C74A}}$ and LC3 when expressed individually or in combination. COS-7 cells were transiently transfected with the indicated constructs and imaged live. (a) Venus is evenly distributed between the cytoplasm and nucleus. (b) Venus-LC3 is enriched in the nucleus relative to the cytoplasm. (c) Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ is enriched in the cytoplasm relative to the nucleus. (d) In cells coexpressing Venus-LC3 and Cerulean-${\mathrm{Atg4B}}^{\mathrm{C74A}}$, LC3 is pulled out of the nucleus. The relative distribution of LC3 between the nucleus and cytoplasm varies between cells depending on levels of ${\mathrm{Atg4B}}^{\mathrm{C74A}}$ present (not shown). (e) In cells coexpressing Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ and Cerulean-LC3, ${\mathrm{Atg4B}}^{\mathrm{C74A}}$ is further enriched in the cytoplasm compared to its distribution in cells expressing Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ alone. Bar, 10 μm.