Fig. 5.

The diffusional mobilities of Venus-LC3 and Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ in both the cytoplasm and the nucleus are significantly slower than those of Venus as assessed by confocal FRAP. (a) Comparison of mean FRAP curves for Venus, Venus-LC3, and Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ in the cytoplasm ($N=30$ cells from 3 independent experiments). (b) Comparison of mean FRAP curves for Venus, Venus-LC3, and Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ in the nucleus ($N=30$ cells from 3 independent experiments). (c)–(e) Representative examples of fits using a one-component diffusion model to the experimentally determined recovery curves for Venus (c), Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ (d), and Venus-LC3 (e) in the cytoplasm (error bars represent 95% CI $N=30$ cells from 3 independent experiments). (f) Diffusion coefficients for Venus, Venus-LC3, and Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ in the cytoplasm and nucleus. Error bars represent 95% CI $N=30$ cells from 3 independent experiments.