Fig. 6.

The diffusional mobility of Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ is significantly slower upon coexpression with Cerulean-LC3, whereas no significant change was observed for the mobility of Venus-LC3 upon coexpression with Cerulean-${\mathrm{Atg4B}}^{\mathrm{C74A}}$. (a) Mean diffusion coefficients for Venus-${\mathrm{Atg4B}}^{\mathrm{C74A,}}$ Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ coexpressed with Cerulean-LC3, Venus-LC3, and Venus-LC3 coexpressed with Cerulean ${\mathrm{Atg4B}}^{\mathrm{C74A}}$in the cytoplasm ($N>20$ COS-7 cells). (b) Mean diffusion coefficients for Venus-LC3 and Venus-LC3 coexpressed with Cerulean ${\mathrm{Atg4B}}^{\mathrm{C74A}}$ in the nucleus. Error bars represent 95% CI with $N=30$ COS-7 cells.