Table 2.

Comparison of the experimentally determined apparent molecular weights to the expected molecular weights for Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$ and Venus-LC3.

Protein	Expected MW (kDa)	Apparent MW (kDa)a
		Cytoplasm	Nucleus
Venus-${\mathrm{Atg4B}}^{\mathrm{C74A}}$	72	$250\pm 80$ (30)	$600\pm 200$ (30)
Venus-LC3	45	$500\pm 100$ (30)	$1800\pm 600$ (30)
Venus-LC3 ($+\mathrm{Cerulean}-{\mathrm{Atg4B}}^{\mathrm{C74A}}$)	117	$400\pm 200$ (30)	$2500\pm 800$ (30)
Venus-${\mathrm{Atg4B}}^{\mathrm{C74B}}$ ($+\mathrm{Cerulean}-\mathrm{LC3}$)	117	$400\pm 100$ (20)	N/A

Values represent the mean $\pm 95\%$ CI. The number of observations is in parentheses.

^aCalculated assuming a spherical shape.