Fig. 2.

Multispectral performance. (a) En face (top) and side-view (bottom) of distal tip of multispectral scanning fiber endoscope with 1.6 mm outer diameter and 10 mm rigid distal tip. Single video-frame image of droplets of peptides labeled with DEAC (blue), TAMRA (green/yellow), and CF633 (red) at (b) 1 μM and (c) 100 μM. Unlabeled peptide shows no signal (only specular reflection). Spectra of the fluorescent-labeled peptides labeled with (d) KCCFPAQ-DEAC, (e) AKPGYLS-TAMRA, and (f) LTTHYKL-CF633 at 1 μM concentration with (dashed lines) and without (solid lines) excitation filters show minimal overlap (crosstalk) among the fluorescence emission bands for the three peptides and small shifts (9 to 14 nm) in the peak emission wavelength of the dye after peptide labeling.