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TRAP-LIG assay was performed using indicated concentrations over each lane with M and D1 (A); D2 and D3 (B). A positive control was run with telomerase, but with no ligand. A negative control was run without either telomerase or ligand. Positive and negative control lanes are indicated by + and – labels, respectively. Other lanes contain TRAP reaction mixtures mixed with the indicated concentrations (µM) of each ligand.
