. 2012 Jun 21;7(6):e39467. doi: 10.1371/journal.pone.0039467

Table 2. Melting temperatures (T _m)^a of Hum₂₄ and Hum₄₈ G4DNA, ligand bound to pre-formed G4DNA and complexes produced when G4DNA was formed in presence of the indicated ligand at [ligand]:[DNA] ratio ‘r’ = 20 for the monomeric and ‘r’ = 10 for the gemini ligands in 100 mM KCl solution.

Entry	ODN/ODN-ligand	Pre-formed G4DNA		G4DNA formed in presence of the ligand
		T _m °C	ΔT _m °C^b	T _m °C	ΔT _m °C^b
1.	Hum₂₄	46	0	46	–
2.	Hum₂₄ + M	52	5	55	9
3.	Hum₂₄ + D1	62	16	70	24
4.	Hum₂₄ + D2	58	12	79	33
5.	Hum₂₄ + D3	68	22	84	38
6.	Hum₄₈	51	0	51	–
7.	Hum₄₈ + M	60	9	65	14
8.	Hum₄₈ + D1	67	16	79	28
9.	Hum₄₈ + D2	69	18	87	34
10.	Hum₄₈+ D3	72	21	89	38

Changes in the circular dichroism spectral peak (at 295 nm for the G4DNA and the pre-formed G4DNA-ligand complex; at 266 nm for the G4DNA-ligand complex formed in presence of ligand) monitored as a function of temperature using 2 µM strand ODN concentration. ^bΔT _m values were obtained from the difference in the melting temperatures of the ligand bound and uncomplexed G4DNA. For the G4DNA-ligand complexes when G4DNA was formed in presence of the ligand, the solution was first heated to 95°C for 5 min and then cooled slowly. The results are average of two experiments and are within ±0.5°C of each other.

Table 2. Melting temperatures (T m)a of Hum24 and Hum48 G4DNA, ligand bound to pre-formed G4DNA and complexes produced when G4DNA was formed in presence of the indicated ligand at [ligand]:[DNA] ratio ‘r’ = 20 for the monomeric and ‘r’ = 10 for the gemini ligands in 100 mM KCl solution.