a. Worm hsf-1. x-axis shows lifespan in days of adulthood. y-axis shows fraction of worms alive. Control refers to transgenic worms expressing unc-119(+); sod-3::mCherry. Worms overexpressing C. elegans hsf-1 live 35% longer than controls (p<0.01, log-rank). b. C. elegans expressing aakg-2(sta2) live 45% longer than controls (p<0.01, log-rank). c. Worms overexpressing C. elegans sod-1 live 25% longer than the control strain (p<0.01, log rank). d. Worms overexpressing C. elegans lmp-2 live 34% longer than controls (p<0.01, log-rank). e. Expression of zebrafish sod1 results in 25% lifespan extension relative to control strain (p<0.01, log rank). f. Expression of zebrafish ucp2 results in 40% lifespan extension relative to control strain (p<0.01, log rank). Overexpression of C. elegans ucp-4 does not show longer lifespan (p>0.05, log-rank). g. Worms expressing zebrafish lyz live 30% longer than control (p<0.01, log rank), whereas expression of C. elegans lys-1 does not result in longer lifespan (p>0.05, log rank). The median lifespan of the control is 18 days. Each experiment was repeated at least three times and representative data from one experiment is shown. Additional data are listed in Table S3.