Figure 4. Caprin1 co-sediments with mFMRP in polyribosomes prepared from total brain and mFMRP is not required for Caprin1 to associate with polyribosomes.

Aliquots containing 10–12 OD at 260 nm were analyzed by sedimentation velocity through sucrose density gradients in the presence of MgCl₂, after incubation with 30 mM EDTA or after treatment with 100 µg/ml RNase A. mFMRP and Caprin1 were revealed simultaneously with mAb7G1-1. 80S : monosomes ; SS : ribosomal small sub-unit ; LS : ribosomal large sub-unit ; L7 : ribosomal large protein 7.