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. 2012 Jun 21;7(6):e39338. doi: 10.1371/journal.pone.0039338

Figure 6. Sedimentation of FMRP and Caprin1 in presence of NaCl at physiological and at high salt conditions. A.

Figure 6

) In presence of 150 mM NaCl, FMRP and Caprin1 are detected associated with polyribosomes. At 400 mM, a clear displacement of FMRP and Caprin1 towards the top of the gradient is observed, while PABP and the ribosomal L7 protein are not affected. In the bottom panels of A) are shown the distribution in the sucrose gradients of CaMKIIα, Map1b and Fmr1, FMRP-mRNA targets in the presence of 150 and of 400 mM NaCl. Note the slight shift toward the lighter fraction in the 400 mM NaCl condition and the absence of mRNA in the fraction containing the highest amount of FMRP at the top of the gradient. The results are presented as percent of the specific mRNA in each fraction. B) In presence of 400 mM NaCl, the majority of FMRP and Caprin1 are found as “floating” species in the loading volume that did not penetrate the gradient even after prolonged ultracentrifugation for 23 hrs. The position of the small ribosomal subunit was determined according to the UV profile and immunoblotting with anti-S6 protein. S values were determined using purified total RNA from E.coli.