Table 5. List of the genes quantified by QRT-PCR analysis.
qPCR Primers | Sequence (5′-3′) |
Total GFAP, forward | AGAAGCTCCAGGATGAAACC |
Total GFAP, reverse | TTCATCTGCTTCCTGTCTATAGG |
GFAPalpha, forward | AGAGGTCATTAAGGAGTCCA |
GFAPalpha, reverse | CAACTATCCTGCTTCTGCTC |
GFAPdelta, forward | CCTACAGGAAGCTGCTAGAG |
GFAPdelta, reverse | GCGTTCCATTTACAATCTGGT |
GAPDH, forward | CTCTCTGCTCCTCCTGTTCGAC |
GAPDH, reverse | TGAGCGATGTGGCTCGGCT |
HPRT, forward | ATGGGAGGCCATCACATTGT |
HPRT, reverse | ATGTAATCCAGCAGGTCAGCAA |
Transcript-specific primers and their sequences for SYBR Green quantitation are mentioned.