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. 2012 Jun 21;7(6):e38609. doi: 10.1371/journal.pone.0038609

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Higuchi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Immunostaining of GFAP after 0, 3, or 14 days of HFD intake in the hypothalamus Abbreviations: 3 V: 3rd ventricle; DMH: dorsomedial hypothalamic nucleus; VMH: ventromedial hypothalamic nucleus. Scale bar: 100 μm. (b) The expression of GFAP after 3 or 14-day HFD intake. n = 6 for each. Results are expressed as the mean ± S.E.M. ^* p<0.05 vs. SD (Student's t-test).