Figure 4. Tangent angle correlation analysis shows that L_p of WT human cardiac αTm equals 40.6−45.8 nm.

ln(<cos(θ)>) data obtained from three separate samples independently prepared under identical conditions are plotted as a function of segment length along the molecular contour. The slope of this plot is –1/2L_p. L_p for WT Tm from this analysis were 45.8±0.8 nm (N = 741, R² = 0.99), 43.5±0.8 nm (N = 628, R² = 0.98) and 40.6±0.8 nm (N = 798, R² = 0.98). The variation in the L_p values represents the uncertainty inherent to our experimental setup and the tangent angle correlation analysis.