Figure 6. Deposition time study suggests L_p measurements were stable with incubation time of 300 s or longer.

L_p values obtained by tangent angle correlation analysis (blue asterisks, left axis) and the corresponding number of molecules considered (black dots, right axis) were plotted against incubation times. An overestimation of L_p was observed at incubation times below 300 s, which may be due to the shorter incubation time and/or smaller number of molecules available for the analysis. L_p measurements were stable at incubation times above 300 s, where variation was comparable to the inherent uncertainty in our methodology. This implies an incubation time of 300 s was sufficient for surface equilibration of αTm on p-Lys coated mica substrate.