Table 1. Summary of Lc, Le-e, and Lp from tangent angle correlation, second moment and end-to-end length analyses.
Incubation time (seconds) | 30* | 300 | 600 #1 | 600 #2 | 600 #3 | 900** |
Number of molecules | 1852 | 199 | 741 | 628 | 798 | 979 |
Contour length, Lc (nm) | 39.3±0.1 | 39.6±0.2 | 39.7±0.1 | 40.0±0.1 | 40.5±0.1 | 40.1±0.1 |
End-to-end Length, Le-e (nm) | 32.8±0.1 | 32.6±0.3 | 33.2±0.1 | 32.9±0.2 | 32.9±0.1 | 33.3±0.1 |
Normalized end-to-end length, le-e | 0.937±0.002 | 0.928±0.005 | 0.932±0.003 | 0.928±0.003 | 0.923±0.003 | 0.930±0.002 |
Persistence length, Lp (nm) by tangent angle correlation | 48.3±0.7 | 42.3±0.4 | 45.8±0.8 | 43.5±0.8 | 40.6±0.8 | 45.5±0.7 |
Persistence length, Lp (nm) by le-e distribution | 64.4±3.4 | 44.7±4.7 | 51.6±2.1 | 49.0±2.8 | 41.7±2.0 | 49.6±3.2 |
Persistence length, Lp (nm) by tangent angle second moment | 45.9±0.3 | 40.9±0.2 | 44.5±0.4 | 41.9±0.3 | 39.5±0.3 | 44.0±0.3 |
0.01% p-Lys deposition on mica by 30 s incubation.
0.01% p-Lys deposition on mica by 300 s incubation.