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. 2012 Jun 15;18:1594–1603.

Figure 6.

Figure 6

YC-1 reduced RGC-5 cell proliferation. A: YC-1 decreased the number of nuclei and the Ki-67 cell proliferation marker expression in RGC-5 cells using Cellomics® high-content screening and the Columbus™ system. RGC-5 cells were treated with 5, 10, 20, and 40 µM YC-1 or 0.066% DMSO for 24 h. After fixation and immunofluorescence staining, cells were detected and analyzed by an HCS reader. Photos show fluorescence staining results of Hoechst 33342 (blue) and Ki-67 (green). B: The quantification results of HCS. * Indicates p<0.05 compared to the Hoechst-stained nuclei of the DMSO vehicle group. # Indicates p<0.05 compared to the Ki-67-stained cells of the DMSO vehicle group.