Table 1. Ultrasensitive viral load measurements.

Macaque	Time (in days)	Viral RNA (copies/mL)	Spike copies original count (RNA copies/mL)
P157	204 and 239 (see Fig. 2)	<3	* 7008
P185	204 and 239 (see Fig. 2)	<3	* 10822
P188	204 and 239 (see Fig. 2)	<3	* 8194
P177	312 and 347 (see Fig. 8)	<3	* 7692
	§591 and 599 (see Fig. 8)	<3	** 6062
	§654 and 658 (see Fig. 8)	<3	** 9060
P252	§215 and 365 (see Fig. 8)	<3	** 6201
4416	238 and 272 (see Fig. 4)	<3	* 8276
BD12	98 and 105 (see Fig. 4)	<3	** 9319
BD53	98 and 105 (see Fig. 4)	<3	** 8494
BD64	400 and 407 (see Fig. 4)	<3	* 18997
BD69	381 and 388 (see Fig. 4)	27	* 8900
	429 and 437 (see Fig. 4)	<4 (less plasma)	** 5569
	451 and 458 (see Fig. 4)	<3	*** 20

Shown are the real time PCR viral RNA measurements of pooled plasma samples (two pooled samples per measurement; total plasma volume 6 mL; detection limit = 3 RNA copies/mL). The time points selected for the analyses are shown as days from the zero point adopted in figures 2, 4 and 8. In these time points, the macaques were under H-iART (unmarked), or off-treatment after therapy suspension (marked with “§”). As a control of the assay variability and to exclude PCR inhibition, spiked RNA measurements for each plasma sample are shown. The RNA copy numbers (in copies/mL) used for the spiked measurements were: * 8686; ** 8605; *** 34. The low copy number spike was chosen to double-check the absence of low-level viremia in macaque BD69 that was the only one showing detectable viral RNA during the first PCR run.