Fig. 2.

Invasive squamous cell carcinoma in the dermis. Histopathology with an approximately 4× field of view; (a) shows a large invasive tumor focus marked by the asterisk (*) as well as an eccrine gland in the dermis (E.G.). In the lower left of the image, collagen and elastin fibrous material can be seen. The 4× histology had resolution of about 5 µm resolution when displayed on a typical computer screen. The corresponding confocal sub-mosaic (b) shows excellent correlation. An additional smaller tumor is marked by a smaller asterisk (*). The smaller tumor is digitally magnified (and slightly rotated for display) in reflectance mode to show structure (c), in acridine orange fluorescence mode to show nuclei (d) and eosin fluorescence-mode to show cell bodies (e). The composite image (f) correlates with optically zoomed histopathology (g) and shows cellular resolution at 30×, which is the key pathological feature.