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. 2012 Jun 22;3:82. doi: 10.3389/fendo.2012.00082

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Copyright © 2012 Ayoub, Al-Senaidy and Pin.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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GPCR-G protein preassembly or agonist-induced association. (A) In the preassembly model, as shown for PAR1-Gαi1 interaction (Ayoub et al., 2007, 2010), a basal BRET signal is measured between the receptor-YFP and Gα-Rluc fusion proteins. Agonist stimulation induces BRET changes (here indicated by an increase) without changing the receptor-G protein interaction supporting agonist-induced conformational changes within the preassembled complexes. (B) The other situation is illustrated by the data obtained with PAR1-Gα12 interaction (Ayoub et al., 2010) where there is no significant basal BRET signal indicating no preassembly and only receptor activation promotes receptor-G protein association and thereby induces BRET signal increase.