FIGURE 5.
Genetic interaction between betaInt-nu and rac1/rac2 in phagocytosis of S. aureus. An assay for phagocytosis in vitro was conducted with FITC-labeled wild-type S. aureus as targets and hemocytes prepared from larvae of control flies (w1118) (A), elmo null flies (elmoKO) (A), flies heterozygous for betaInt-nu2 (betaInt-nu2/+) (B), flies heterozygous for rac1 and rac2 (Rac1J11 Rac2Δ/+) (B), or flies trans-heterozygous for betaInt-nu2, rac1, and rac2 (betaInt-nu2/Rac1J11 Rac2Δ) (B) as phagocytes. Genotypes of the fly lines used are elmoKOFRT40, 42/elmoKOFRT40, 42 (elmoKO) and y1 w*; Rac1J11 Rac2Δ FRT2A/TM6B, Tb+ (Rac1J11 Rac2Δ).