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. 2012 May 10;287(26):21717–21728. doi: 10.1074/jbc.M112.369496

FIGURE 3.

FIGURE 3.

VH1 domain of CTN-1 is critical for CTN-1 localization and function. A, representative GFP images from the muscle cells of C. elegans transgenic animals expressing GFP-tagged full-length CTN-1 or deleted forms of CTN-1. CTN-1Δ1–279 lacking the VH1 domain is localized primarily in the cytosolic compartment. CTN-1Δ551–784 lacking the C-terminal region shows a diffused punctate pattern, whereas the expression pattern of the CTN-1Δ382–416 construct that lacks the coiled-coil domain is similar to that of the full-length construct. Scale bar is 10 μm. B, first curvature angle obtained from wild-type (WT), ctn-1(eg1167) mutant, and several transgenic ctn-1 rescue strains. Error bars, S.E. n.s., not significant (p > 0.05). *, p < 0.01, unpaired Student's t test, n >5. Representative photos show the anterior portion of wild-type, ctn-1, and ctn-1 transgenic rescue strains. White circles indicate the four most anterior of the 13 midline points for a wild-type animal (21). The first curvature angles are angles between two white lines resulting from the first three dots.