FIGURE 5.

ERK activation is mediated by effects on TORC1- 8226 cells were transfected with shRNA targeting scrambled sequences (control;scr), or shRNA targeting two separate sequences in rictor (ric 1 and ric 2) or raptor (rap 1 and rap 2). In A, control or rictor knocked down clones treated ± pp242 for 30 min and, in B, control or raptor knocked down clones treated. Following treatment, immunoblot assay performed for phospho-ERK, total ERK, rictor, or raptor. Fold increase is ERK-P/ERK-T densitometry ratio compared with control, non-pp242 treated cells for each of the 6 separate clones. * denotes significant increase (p < 0.05) in ERK-phosphorylation induced by pp242.