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. 2012 Apr 17;287(26):21904–21913. doi: 10.1074/jbc.M111.315200

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — ATM-conditioned medium induces lipid accumulation in human cancer cells. T47D (A, B, and E) and HT-29 (C and D) cells were treated for 72 h with ATM or MDM-unconditioned medium (UC) or CM, and mRNA levels of CD36 (A and C) and fatty acid synthase (FAS; B and D) were analyzed by Q-PCR and normalized to cyclophilin mRNA. Results are expressed relative to the levels of cells in the presence of ATM or MDM unconditioned medium set at 1. Each bar is the mean value ± S.D. of triplicate determinations. Statistically significant differences are indicated (*, p < 0.05; **, p < 0.01; ***, p < 0.001). E, Oil Red O staining performed on T47D cells. Results represent a single experiment repeated four times with similar results.