FIGURE 4.

PCM induces an ATM-like phenotype in MDM. MDM from lean subjects were treated with PCM or unconditioned PGM during 72 h (A). mRNA levels of CXCL1, CXCL5, CXCL9, CXCL10, CXCL11, CXCL12, MCP-1, TNFα, and IL-1b were analyzed by Q-PCR and normalized to cyclophilin mRNA. Results are representative of three experiments and are expressed relative to the levels of cells in the presence of PGM set at 1. Each bar is the mean value ± S.D. of triplicate determinations. Statistically significant differences are indicated (***, p < 0.001). MDM were treated with PCM for the indicated time periods (B). Western blot analysis was performed using antibodies against total or phosphorylated forms of Stat3, Stat1, and p44/p42 MAPK (ERK1/2).