FIGURE 3.

Overexpression of FKBP8 increases the stability of ΔF508-CFTR at physiological temperature. Immunoblot analysis of CFTR, Hsp90, Hsp70, and FKBP8 following lentiviral expression of FKBP8 in CFBE41o− cells expressing ΔF508-CFTR cultured at 37 °C (A) or WT-CFTR (C) or ΔF508-CFTR cultured at 30 °C (E). Quantitative analysis of ΔF508-CFTR band B (B) and C (C) glycoforms following overexpression of FKBP8 in CFBE41o− cells expressing ΔF508-CFTR cultured at 37 °C (B), or WT-CFTR (D) or ΔF508-CFTR cultured at 30 °C (F). B, D, and F data were normalized to the percent of the maximal signal. Data are shown as a mean ± S.E., n ≥ 3. p values were determined by two-tailed t test using the control as the reference point; asterisks indicate p < 0.05.