FIGURE 4.

Overexpression of hFKBP8 in yeast increases the stability of CFTR. Quantitative analysis of WT-CFTR remaining over time under mock transformed conditions (closed squares) or following expression of hFKBP8 (open squares) is shown. FKBP8 expression was induced with copper sulfate for 4 h and subsequently stopped with cycloheximide prior to analysis of CFTR stability. The data were expressed as the fraction of CFTR remaining at t = 0. Data are shown as a mean ± S.E., n ≥ 3. p values were determined by two-tailed t test using the mock transformed yeast as the reference point; asterisks indicate p < 0.05. A.U., arbitrary units.