FIGURE 4.

Fpr1 is secreted. A, Western blot analysis of cell lysate and culture supernatant of the F. oxysporum wild-type strain. Upper panel, samples separated by SDS-PAGE and stained with Coomassie Blue. Lower panel, immunoblot analysis with α-Fpr1 antiserum. B, Western analysis of culture supernatants of the wild-type strain grown on liquid minimal medium in the absence (MM) or presence of tomato roots (Root) or on potato dextrose broth (PDB). Relative positions of molecular weight markers are indicated at the left.