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Characterization of chimeric GSHKT16 protein. A, denaturing gel electrophoresis of the recombinant GST and GSHKT proteins. B, gel filtration of GST (upper) and GSHKT (lower) on Superdex-75. C, far-UV CD spectra of GST (black line) and GSHKT (red line) acquired using a 1-mm path length quartz cell at 25 °C. Protein concentrations were ∼0.25 mg/ml in PBS. deg, degrees.
