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. 2012 May 8;287(26):22287–22294. doi: 10.1074/jbc.M112.345884

FIGURE 5.

FIGURE 5.

RTP1S C-terminal half is important for protein-protein interaction with ORs. A, left panel, interactions between Olfr599 and RTP1S deletion mutants. Right panel, interactions between Olfr599 and RTP1S chimeras. First and second panels, protein lysates of HEK293T cells transfected with FLAG-tagged Olfr599 and/or HA-tagged RTP1S deletion mutants and chimeras and blotted with anti-HA or anti-FLAG antibody. Third panels, co-immunoprecipitation of certain RTP1S deletion mutants and chimeras with anti-FLAG antibody. Fourth panels, co-immunoprecipitation of Olfr599 with anti-HA antibody when transfected with certain RTP1S deletion mutants and chimeras. Refer to supplemental Fig. S5 for the original blots. IB, immunoblot; IP, immunoprecipitation. ANP32B is a presumably non-interacting protein, and pCI is the empty vector. B, quantification of the co-immunoprecipitation assays with Olfr599 and RTP1S deletion mutants and chimeras in A. Immunoprecipitation efficiencies were calculated by dividing by the input amounts of both components and then normalizing to the interaction between Olfr599 and wild-type RTP1S. The results shown represent the mean ± S.D. from two independent experiments. Note that Ch4 presented unusually large ratios due to a small denominator (a small amount of proteins in protein lysates).