FIGURE 2.

Chronic Cch treatment boosts Arc protein expression via a transcription-dependent mechanism. A, representative Western blots and quantitative analysis show Arc protein expression in cells treated with Cch for 2, 10, 30, 60, or 120 min (black boxes) followed by washout (white boxes) with or without atropine until lysate preparation. All lysates were prepared 120 min after beginning the Cch treatment. Arc levels are normalized to GAPDH and expressed in percent of Arc expression induced by chronic Cch treatment. Asterisks indicate significant increase relative to Ctrl (p < 0.05; n = 5). B, representative Western blot shows expression of Arc protein in SH-SY5Y cells stimulated with Cch for 30 or 120 min. Cells stimulated for 120 min additionally received ACD or vehicle 30 min after the onset of stimulation (Ctrl, unstimulated cells).